Synthesis of poly (amidoamine) (PAMAM) dendrimer-based chitosan for targeted drug delivery and cell therapy

Synthesis of poly (amidoamine) (PAMAM) dendrimer-based chitosan for targeted drug delivery and cell therapy

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Introduction: In the current study we designed a sophisticated drug delivery nanoparticle to control the methylprednisolone succinate delivery rate and affect the cancer cell growth in culture condition.Materials and methods: Magnetic nanoparticles were synthesized through co-precipitation method.Fe3O4 was first prepared via co-precipitation method and then its surface was functionalized with polyamidoamine (PAMAM) nanodendrimer.

PAMAM synthesis trait was detected via FT-IR and SEM Yeti methods.Methylprednisolone drug was loaded on PAMAM@Fe3O4 and its effect against cancer cell lines was studied.In order to slow down drug release rate from nanoparticles, PAMAM@Fe3O4 were coated with trimethylchitosan (TMC) after drug loading.

Performance of PAMAM@Fe3O4@TMC nanoparticles loaded with mmethylprednisolone, were evaluated against two cell lines to detect the cytotoxic and apoptotic effects by invert light scanning microscopy, immunoassay, and LDH cytotoxicity Kit.Results: According to SEM, image size of Fe3O4 was 4.79-6.

37nm, which is smaller than nanodendrimer (6.30-43.67 nm).

FT-IR spectrum for ester bond Methylacrylate @ Ethylendiamin was obtained to be 1720-1730 cm-1.FT-IR Spectrums 600 cm-1, 1000 cm-1 belong to Fe3O4, and Fe3O4@ NH2.Also, trimethyl chitosan coated Nanoparticle @ Drug, smearing trimethyl chitosan with Glutaraldehyde, created cross link between TMC monomer at low drug doses in each complete nanoparticle, gave confidence drug side effect, therefore, this nanoparticle FENU-THYME could be safe for future cancer therapy.

Conclusion: The results showed that drug delivery via PAMAM@Fe3O4 nanoparticle reduces cell viability in vitro condition.